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GGTC German Gene Trap Consortium

 

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 FAQs

 

 

 

1. Are gene traps knockouts?

Intronic gene traps produce fusion proteins, which may or may not have residual function. In general, the more 5' the insertion the more likely is a knock-out. In addition posttranscriptional skipping of the GT vector has been reported on occasion, which may result in the expression of wildtype transcripts to various degrees. In such cases a hypomorphic phenotype or no phenotype at all can be observed.

 

 

 

2. What is the germline transmission rate of the cells?

In our hands, the transmission frequency is currently 70% for TBV-2 and 40% for E14 ES cells with the exception of the "F" lines, from which so far less than 30% went germline.

 

 

3. how to find gene x in the database if I cannot find it by keywordsearch?

Sometimes keyword searches are unsuccessful because our stringency is too high. The "e" value is based on different parameters like the size of the database, the length of the query sequence and the identity. Since we have mouse sequences but many hits in the database are derived from human or from other organisms, you might want to reduce the "e" value as well as the percentage of identity.

 

 

 

4. I found my gene of interest three times in the database. One clone ID is W047B06 the other is W047B07 and another is W037C05. Does it make sense to inject them all?

In principle, only one of the W047 - clone needs to be injected, since these cells are derived from the same plate ("W047") and are probably derived from the same integration event (check the RACE results if you are in doubt). Clone W037C05 on the other hand is independent from the other integration.

 

 

5.How do I get information about the vector and the cell line that was used?

Click the clone ID again.


 

6. Can I obtain cells exclusively?

No.

 

 

7. Can I distribute your cells to third parties?

According to our Material Transfer Agreement, no cells shall be distributed to third parties without our permission. However, as a rule for academic research, permission will be granted on request.

 

 

8. Are there any pathogens in the cell lines, that might be transferred to other cells or mice?

All GGTC ES cell lines, which are sent out, are tested for mycoplasma infection and found not infected.

 

 

9 . What is the genotype of tbv-2 cells?

These cells are 129S2 (formerly 129/SvPas) and were originally established at the GSF in Neuherberg by Veronique Blanquet.

 

 

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